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KMID : 0900919990230010029
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Korean journal of Animal Reproduction
1999 Volume.23 No. 1 p.29 ~ p.36
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Effects of MethyIxanthine Derivatives on Induction and Enhancement of Hamster Epididymal Sperm Motility
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Park Y.-S.
Song S.-J.
Lee H.-J.
Kim N.-H.
Lee H.-T.
Chung K.-S.
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Abstract
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This study were performed the effect of methylxanthine derivatives on hamster epididymal sperm motility. To assess the effect of methylxanthine derivatives on motility kinematics of epididymal sperm, pentoxifylline, 2-deoxy-adenosine and hypoxanthine were added to TALP medium. 1 mM of pentoxifylline, significantly increased VCL, VAP, VSL of spermatozoa obtained from corpus and cauda epididymis. With 1 mM of 2-deoxyadenosine, VCL, VAP, VSL of spermatozoa obtained from corpus and cauda epididymis were significantly increased, but 2 mM ADE for cauda spermatozoa was effective than 1 mM. In the case of hypoxanthine, various concentrations were not significantly effective, but 2 mM HX showed higher effect than other concentrations. pentoxifylline 1 mM and 2-deoxyadenosine 1 mM significantly increased VCL, VAP of corpus and cauda epididymal spermatozoa and VSL of cauda epididymal spermatozoa. From these results it could be concluded that the addition of methylxanthine increase motility kinematics of hamster spermatozoa collected from epididymis.
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KEYWORD
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Pheasant, Measurement, Image analyser, Spermatogenic cell
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